UV-Vis spectroscopy is a method to measure the absorption of visible light (390 nm to 800 nm) and UV light (190 nm to 390 nm). The substances are measured in solution contained in a quartz cuvette.
Using a spectrometer, light with a wavelength range of 190 nm to 800 nm is radiated through the cuvette and absorption spectrums are recorded. Different broadband UV-Vis light sources can be used.
Mercury lamps that emit just a single line spectrum are also used for the wavelength calibration or detecting mercury.